In the PulseNet and TESSy network, regular ringtests are performed to ensure the profiles from the different labs can be compared. PFGE is a technique with a lot of variation and even a different brand of agar can cause differences in the profile. You should be very aware that this only has a scientific value if the same protocol was used to obtain the profiles. However, it is possible in BioNumerics to use the database exchange tools to send your profiles to other researchers (with BioNumerics) or vice versa and compare them to each other. aureus there is no initiative to collect PFGE profiles into a central database. coli and Mycobacterium tuberculosis are being monitored like this. Currently in Europe, only Listeria, Salmonella, E. However, these networks are mainly aimed at foodborne pathogens. This data can be submitted to the TESSy network of the ECDC (access is restricted to the national reference labs). In Europe, the same protocol is also being used by most reference labs. In the US, all CDC state labs perform PFGE typing according to these protocols and submit their data to a central database for comparison. Molecular clusters are generated using bespoke software or packages such as BioNumerics (to rapidly detect clusters and. A typical protocol that is used worldwide is the PulseNet protocol, developed by CDC. PFGE profiles can only be compared between labs if they have been made with the same, very standardized, protocol.
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